Diagnosis Legionella sp: Kultur dan Pewarnaan
Keywords:
Legionella, Dieterle, BCYE, PneumoniaAbstract
Legionella adalah bakteri batang gram negatif, tidak berspora, tidak berkapsul yang biasa ditemukan pada lingkungan air tawar, sistem air buatan manusia, tanah yang lembab, dan juga bahan kompos. Legionella disease paling banyak disebabkan oleh Legionella pneumophila serogroup (Sg) 1 dengan angka kejadian menyentuh angka 90% dari total kasus. Sampel untuk pemeriksaan legionellosis menggunakan sputum atau sekresi pernapasan, sampel jaringan, darah, serum, dan urin dengan gold standard berupa kultur sputum, hal ini dikarenakan kultur memungkinkan untuk identifikasi secara spesifik spesies, serogroup, dan uji sensitivitas antibiotik. Legionella terlihat lebih jelas dengan pewarnaan Giemsa dan pewarnaan Silver seperti Dieterle dan Warthin-Starry dibandingkan dengan pewarnaan Gram. Bakteri Legionella dengan pewarnaan Dieterle diberi nilai +3, hal ini menunjukkan hasil sangat jelas ditunjukkan dengan bakteri berwarna hitam dengan latar belakang berwarna kuning-kecoklatan.
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