Perbandingan Bright Field Microscopy dan Phase Contrast Microscopy dengan Pengecatan Lactophenol Cotton Blue pada Jamur Dermatophytes
Keywords:
Helicobacter pylori, Haematoxylin & Eosin (H&E), Toluidine Blue, Warthin-StarryAbstract
Dermatophytes adalah jamur berfilamen yang menyebabkan infeksi jamur superfisial pada kulit, rambut, dan kuku dengan cara menginvasi dan memakan jaringan yang berkeratin. Penelitian ini dilakukan untuk membandingkan antara Bright Field Microscopy (BF) dengan Phase Contrast Microscopy (PC) untuk melihat jamur Dermatophytes yang diwarnai Lactophenol Cotton Blue (LCB). Sampel penelitian berupa kerokan kulit yang ditanam pada media Sabouraud Dextrose Agar (SDA) yang diberi antibiotik berupa gentamicin dan amoxicillin, kemudian dilakukan inkubasi pada suhu ruang selama kurang lebih 2 - 4 minggu. Jamur yang dilihat dengan mikroskop BF diberi nilai +2 karena struktur jamur dapat terlihat dengan jelas namun tidak bisa memberikan kontras yang baik, sedangkan mikroskop PC diberi nilai +3 karena selain dapat melihat morfologi jamur dengan jelas juga dapat memberikan kontras yang lebih terang antara jamur dan latar belakang.
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